The experiment was conducted with an aim to compare the gross and histomorphology of the epididymis of pre-pubertal, pubertal and adult indigenous bulls (Bos indicus) of Bangladesh. The tissue samples (testes with epididymes) were collected from nine (09) bulls of three age groups namely pre-pubertal (1.5 to 2 years), pubertal (2 to 3 years) and adult (above 3 years) from the Govt. slaughter house, Sylhet, Bangladesh immediately after slaughter and were grossly examined, measured for weight or length and processed for histology. Grossly, three major segments of the epididymis were revealed namely the head, body and tail. The oval shaped head was connected to the U shaped tail by a narrow slender body of the epididymis. The length and weight of left epididymis was slightly larger than the right epididymis and epididymal tail was the largest of the three segments in all three studied groups. Histological observations revealed that the epididymis was characterized by epididymal ducts separated by connective tissue and surrounded by the peritubular smooth muscle layer. Luminization started from the head of the prepubertal stage. A variety of cell types were observed in the lining epithelium extending into lumen including the principal cells, basal cells and apical cells. The head was characterized by tall pseudo-stratified columnar epithelium with apical cells extending into lumen, but no sterocillia was observed in that part of epididymis. The lining epithelium in the body was low columnar epithelium with short narrow cells and stereocilia. The amount of spermatozoa was comparatively higher in the lumen of the body and tail region respectively. Histomorphometric result showed that there was a gradual reduction in epididymal epithelial height of the tail compared with the head of the right and left epididymis. It was concluded that the studied animals exhibited interesting morphological features of epididymis in three age periods of indigenous bulls of Bangladesh. These basic morphological studies can be important for further studies related to reproductive biology and breeding
