Malaria is one of the most common vector-borne diseases in widespread in tropical and subtropical regions, including part of the Asia and Africa. It is the world’s most dreadful tropical disease. The aim of this study was to investigate the larvicidal activity of acetone, benzene, chloroform, hexane and methanol leaf extract of Oxystelma esculentum against Anopheles stephensi. Twenty five early third instar larvae of An. Stephensi was exposed to various concentrations (30- 180ppm) and was assayed in the laboratory by using the protocol of WHO 2005; the 24h LC50 values of the O. esculentum leaf extract was determined by probit analysis. The LC50 and LC90 values of acetone, benzene, chloroform, hexane and methanol leaf extracts of O. esculentum against An. Stephensi larvae in 24 h were 75.46, 68.55, 98.47, 88.24, 63.84 and 140.66, 130.65, 184.10, 169.36 and 122.48 ppm, respectively. From the results it can be concluded the crude extract of O. esculentum as an excellent potential agent for controlling An. Stephensi mosquito
