Different soil samples were collected for the isolation of PHB producing bacillus sp in and around Coimbatore area. As the preliminary analysis the screening, extraction and estimation of PHB, Physical optimization of PHB production, chemical characterization of extracted powder was done by FTIR analysis. Ampicillin is the drug of choice for per oral administration using nanoprecipitation technique. The production of Poly-β-hydroxybutyrate (PHB) as nanoparticle containing Ampicillin, increase the stability of loaded drug. The extracted PHB created as nanoparticles and the nano encapsulation was done by PCL immobilization method with presence and absence of drug. The resulting nanoparticle is characterized by scanning electron microscopy (SEM) analysis.
