The present study was designed to evaluate the neuropathological effect of Bayluscide, Selecron and ethanolic extract of Anagalis on the neurons of the cerebral ganglia in the freshwater snail B. alexandrina. The snails were subjected to lethal concentrtion (LC90) of each compound (3.468 ppm for Selecron, 0.082 ppm for Bayluscide and 38.129 ppm for ethanolic extract of A. arvensis) till death of all snails after 90 minutes. Then the snails were dissected and the cerebral ganglia were removed. Electron microscopical examination of treated animals revealed severe ultrastructural alterations in the cerebral ganglia. These alterations included hyperchromatic, pyknotic or highly shrunken nuclei, extreme indentation of plasma membrane, atrophy of the perikarya of some neurons, margination of nucleoli, fragmentation or dilation of rough endoplasmic reticulum, damage of mitochondria and vacuolation and destruction of cytoplasm. In addition, degenerated synaptic vesicles and increased number of autophagosomes and myelin figures were frequently observed.
The present study was designed to evaluate the mode of action and neuropathological effect of Selecron, Bayluscide and ethanolic extract of Anagalis arvensis on the neurons of the cerebral ganglia in the freshwater snail B. alexandrina. The snails were subjected to lethal concentration (LC90) of each compound (3.468 ppm for Selecron, 0.082 ppm for Bayluscide and 38.129 ppm for ethanolic extract of A. arvensis) till death of snails after 90 minutes. Then the snails were dissected and the cerebral ganglia were removed. Electron microscopical examination of treated animals revealed severe ultrastructural alterations in the cerebral ganglia. These alterations included hyperchromatic, pyknotic or highly shrunken nuclei, extreme indentation of plasma membrane, atrophy of the perikarya of some neurons, margination of nucleoli, fragmentation or dilation of rough endoplasmic reticulum, damage of mitochondria and vacuolation and destruction of cytoplasm. In addition, degenerated synaptic vesicles and increased number of autophagosomes and myelin figures were frequently observed
In the present study the acetylcholinesterase enzyme (AChE) activities was measured in B. alexandrina snails exposed to the same concentration of the tested compounds. The AChE activities in B.alexandrina showed wide variation along the treated snails and control snails, The AChE activities in B. alexandrina decreased significantly at bayluside bayluside (–55.3% reduction) followed by Selecron (– 49.2% reduction), and A. arvensis (–39.9% reduction).
