An experiment was conducted to study the effect of methyl jasmonate (Me-JA) seed priming on osmolytes and vitamins in 15 days old seedlings of C. cajan under copper stress. Me-JA treated C. cajan seedlings were grown in petriplates lined with Whatman filter paper containing different concentration of copper sulphate (Cu) in seed germinator for 15 days. After 15 days proline, glycine betaine, vitamin A, vitamin B2 , vitamin C and vitamin E were studied. Proline accumulation was increased to 430% in seedlings grown in 5mm Cu with Me-JA treatment and increased to 514.2% in 1µM Me-JA treated seedlings grown in 1mM Cu stress. Similarly glycine betaine content was increased to 103.4% in 1pM Me-JA treated seedlings grown in 5mM Cu stress. Vitamin A content was increased to 44.01% in 10mM Cu as compared to the control which was only 20.3% in 1µM Me-JA treated seedlings grown in 10mM Cu stress. Vitamin B2 was however decreased in all the treatments. Vitamin C content was also increased to 224% in 1pM Me-JA treated seedlings grown in 10mM Cu stress. it was concluded that Me-JA help plant in adjusting to Cu stress condition by increasing proline , glycine betaine and vitamin content in C. cajan seedlings.
