Organochlorine (OC) pesticide residues in pooled water of paddy field samples of Prakasam District have been investigated to find out the extent of pesticide contamination and accumulation. Collected Water samples were processed using a liquid-liquid extraction followed by Gas-Chromatographic technique for pesticide residue quantification with mass selective detector (GC-MS). The conditions were followed for GC, Column: HP-5MS, 30m x 0.25mm x 0.25 μm 50% Phenyl Methyl Siloxane, Injection Temp: 260 0C, Injection Vol. 1μl, Column flow: Helium 1mL/min, Oven hold up time 2.0 min. For MS, Mass spectrometer with single Quadrupole, Operation in SIM Mode, MS Source:230 0C, MS Quad: 1500C Solvent Delay: 3.00 minutes and GC-MSD detecter were maintained. Eight OC pesticides namely α-HCH, β-HCH, δ-HCH, γ-HCH, Dieldrin, β-Endosulfan, p,p-DDD and o,p-DDT were traced. In the pesticide residues, highest β-Endosulfan (0.000082 mg/L) followed by γ- HCH (0.000418 mg/L), o,p-DDT (0.000228 mg/L), α-HCH (0.000082 mg/L), δ-HCH (0.000067 mg/L), p,p-DDD (0.000062 mg/L), β-HCH (0.000040 mg/L) and Dieldrin (0.000025mg/L) were traced in analyzed water samples. The LOD and LOQ were calculated as 0.000006 mg/L and 0.00002 mg/L.
