A non-experimental descriptive study was carried out to assess the knowledge on prevention of urinary tract infection among adolescent girls in selected nursing college. Sample size for the present study consists of 30 adolescent girls from the Aswini College of nursing. Probability random sampling technique was adapted for the selection of sample. The tool was prepared by reviewing literature based on that standardized questionnaire was prepared. The present study reveals that out of 30 samples 93% have average knowledge, 7% have inadequate knowledge and no one have adequate knowledge. There is no association between knowledge of prevention of urinary tract infection and selected demographic variables like monthly income, area of residence, type of family, history of urinary tract infection.