Objective
To observe relationship of optimal endometrial thickness with change in interleukin (IL-Iβ), estradiol and progesterone from follicular to luteal phase in females of intra cytoplasmic sperm injections
Subjects and Methods
A cross sectional survey of564females of ICSI was carried out from July 2011 to November2013.Receiver operating curve identified 8 mm endometrial thickness for implantation and clinical pregnancy in these females. They were categorized into group I and II on the basis of endometrial thickness less than and greater than cut off value (8 mm) respectively. Estimation of hormones estradiol (E2) and Progesterone (P) with interleukin I-β (IL-I-β)was done in follicular and luteal phase by enzyme linked Immunosorbent assay.
Results
Study comprised of 232 (41%)and 332 (59%) females in Group I and II respectively. The P levels were significantly lowin group II (pregnant females with optimal endometrial thickness) in both phases (p<0.0001). On the other hand, E2 was less in follicular phase and more in luteal phase of group II when compared to non-pregnant females (p<0.0001). IL- Iβ was greater infollicular and less in luteal phases of group II females (p<0.0001).
Conclusion
In females for optimal endometrial thickness for implantation, increased E2production in luteal with respect to follicular phase and perseverance of low P levels was observed. A significant high ratio of IL-Iβ from follicular to luteal phase in these females highlights the role of immune mechanisms together with changes in hormonal parameters for endometrial receptivity required for implantation.
