Global risk of petroleum based non- degradable plastics and solid wastes produced from different industries have increased in an alarming rate urging for an effective alternative. The present work focuses on the production of Polyhydroxyalkanoates (PHA) utilizing coffee husk as a cheap organic substrate. PHA producing bacteria was isolated from coffee husk dumped soil and screened in Nile blue agar plates, they were subjected to 16SrRNA sequencing. The organism was further inoculated into two different media - Simple Media and Mineral Salt Media. Further coffee husk with media components was compared with coffee husk extract with different percentages of glucose (2% and 4%). The ability of the organism to utilize coffee husk alone without any media supplementation was also studied. PHA was extracted by Sodium hypochlorite method and quantified by UV spectrometry. FT-IR analysis was done to authenticate PHA production.
