Pectinases are group of enzymes that attack pectin or pectic acid and depolymerize it by hydrolysis and transelimination, endopectinases acts on pectin or pectic acid in a random cleavage mechanism. Endopectinases are Endo PG, Endo PMG, Endo PL and Endo PAL. Six fruit rot fungi isolated and tested for endopectinases production by using pectin/pectic acid as a substrates in Asthana and Hawker’s medium (In vitro) and for In vivo studies six fruits were used i.e., Apple, Mango, Tomato, Sapota, Grape and Orange. In vitro studies the maximum endo PG was noticed in Aspergillus flavus during its 14 days of incubation (52.6 RVU) endoPMG was (50.0 RVU) by Rhizoctonia solani during its 14 days of incubation. Endo PL was 22.2 RVU by Penicillium citrinum after 7 days and Endo PAL was 55.5 RVU Penicillium citrinum after 14 days. In In vivo studies the maximum endo PG was recorded in Mango during 6 days (66.7 RVU) by Rhizoctonia solani, R. stolanifer, A flavus and after 4 days in P. citrinum, M.racemosus while endo PMG was noticed in A. niger (62.4RVU) in mango fruit pulp after 6 days. Endo PL was maximum in M.racemosus (51.8 RVU) while the endo PAL was maximum (66.7 RVU) showed by M.racemosus also. While A. flavus and A.niger after 4 days in Mango fruit pulp. Among these 6 fungi P. citrinum, M.racemosus and A. flavus were the potential strains in the endopectinases production and most useful in fruit juice technology
