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Morinda citrifolia, commonly referred as ‘Noni’ belongs to Rubiaceae and is known for its high medicinal values. The present work aimed to determine the phytochemical studies, the total phenolics, flavonoid, tannin and Vitamin C estimation and to assess the antioxidant properties and antibacterial activities from various extracts of flower, matured and ripened fruits of M. citrifolia. Among all the extracts methanol extracts of ripened fruit noticed phytochemicals such as carbohydrates, proteins, alkaloids, steroids, terpenoids, saponins total phenols, flavonoids and tannins. Highest content of ascorbic acid (Vitamin C) was observed in the ripened fruit extract. All the extracts showed moderate to potent antioxidant activities, among which methanol extract of ripened fruit exhibited 78% and IC50 with 0.204 mg/mL. Thus, revealed to be the strongest antioxidant activity almost nearer to standard ascorbic acid and thus could be a potential rich source of natural antioxidant. In antimicrobial screening, methanol extracts of ripened fruit showed the highest mean zone of inhibition ranging from 9.0 to 13 mm against tested microorganisms. All these experimental finding suggests that the ripened fruit can be the best source to develop pharmaceutical products for the benefit of human health.