Coleus forskohlii Briq. Is a useful medicinal herb in India. Coleus is used to treat hypertension, rheumatism, bronchitis, whooping cough, indigestion, epilepsy and dyspepsia. The present study was aimed to investigate the antimicrobial activity of ethanol extract of root of Coleus forskohlii was evaluated by disc diffusion method against some microbial species like Staphylococcus aureus, Pseudomonas aeruginosa, E.coli, Bacillus subtilis and Aspergillus niger. Staphylococcus aureus is the most sensitive strain (12.03 mm) of those tested with ethanolic extract of root of Coleus forskohlii. Whereas other microorganisms were showed less antibacterial sensitivity to the tested extracts. However, this study revealed maximum growth inhibition and effectiveness was remarkably observed in the extracts of Coleus forskohlii.