L-asparaginase plays a vital role in medical application, particularly in treatment of acute lymphoblastic leukemia as an effective anti-tumor agent. The present study aims to isolate and identify asparaginase producing microorganism present in clay factory waste water and to evaluate the biological activities such as antimicrobial, hemolytic and anticancer activity exhibited by the bacterial strains. This study also involves optimization of L-asparaginase production under solid state fermentation using agro by products such as coconut oil cake, cotton seed oil cake and groundnut oil cake. The present study reveals that Moraxella sp isolated from clay factory waste water showed the highest asparaginase and hemolytic activity. On optimization maximum asparaginase enzyme activity was shown by coconut oil cake. Present study also revealed that the asparaginase enzyme collected from species exhibited potent cytotoxicity against A549 cell line
