Xylanases are of great significance with tremendous potential to offer the industry. These are one of the major enzymes of the commercial sector, especially in paper and pulp industry. Major applications of xylanases also include bioconversion of lignocellulosic material and agro-wastes to fermentative products, clarification of juices, improvement in consistency of beer and the digestibility of animal feed stock. Therefore in the present study the production of xylanase by the fungus Gleomastix indicus under solid-state fermentation (SSF) was investigated. Optimal xylanase production (99.96 U/ml) was obtained using wheat bran as the solid substrate with 96 hours, pH 8, 28 ± 30 C, 20% substrate concentration and inoculum level of 20% (w/v). Galactose and potassium nitrate are the best carbon and nitrogen sources respectively