The aim of the present study was to determine the relationships between milk yield with some environmental factors and physiological characters of Zavot cows using path analysis. It was carred out in 44 smalholders that existed 36-168 old age cows in north-east Turkey. Milk yield (Y) negative correlated with animal age (X1) (-0.021). But, it (Y) positively correlated with lactation period length (X2), number of animals available in enterprise (X3), number of inhabitants in enterprise (X4) (r = 0.550, 0.132*, 0.701 respectively). The path analysis showed that animal age, lactation period length, number of animals available in enterprise had high of direct effects on milk yield (88.84%, 77.90% 83.71% respectively). The effect of number of inhabitants in enterprise via lactation period length on milk yield was found 62.37% and path coefficient was found as -0.0547. Besides, the effect of number of inhabitants in enterprise via number of animals available in enterprise on milk yield was found noteworthy (16.88%, P = -0.0148).