Salinity stress is a major contributor in decreasing crop productivity and threatening agricultural sustainability. In the present study the activity of lipid peroxidation and peroxidase during salt stress treatment for different periods in Vigna mungo and Vigna aconitifolia leaf tissues was done. Seedlings of Vigna mungo and Vigna aconitifolia were grown through Hydroponics system containing Hoagland solution under optimum condition of 70% humidity and temperature (28ºC). MDA contents and activity of peroxidase was determined in the leaves after 7 days of induction under salinity stress. Salt stress led to an increase in peroxidase activity, along with an enhanced accumulation of malondialdehyde (MDA) as compared to control shows variation with increased time duration and with concentration in both plants.