Pharmacopeial standard development, pharmacognosy, hptlc. fingerprinting and physicochemical research studies of beninca hispida (thunb.)

Research Article
*Pawan Kumar Sagar, N. Z. Ahmed, R.P.Meena and S. Kashyap
(Beninca hispida (Thunb.), therapeutic disorders, Development of Pharmacopeial Standards, physicochemical screening, QC, QA.

(Beninca hispida (Thunb.) - Petha is a very important processed, dynamic commercial product found of North India. It is processed largely in North India- Uttar Pradesh, Uttarakhand plan area and Bihar States from wax gourd or ash gourd. Petha sweet is having geographical indication (GI) tag from Agra, U.P. State in India. BH. used as amazing therapeutic potentials as a Antioxidant, Antidiabetic, Hypoglycemic and Hypolipidemic, Anti-inflammatory, Alzheimer’s disease, Anti-compulsive effect, Allergic inflammation, Antibiotic, Anti-microbial, Anti-Ulcer, Anti-Acidic, Anti-asthmatic, Cytotoxic and Anticancer, Anti-obesity, Antidepressant and Anxiolytic, Analgesic and Antipyretic, Antihypertensive, Anti ageing of Skin, Gastro protective from since ancient time. Three fresh samples of BH. taken into these studies from North India Region U.P., U.K. and Bihar State which 3 samples were freshly collected P1,P2 and P3. and developed from applied SOP. on the basis of authenticated classical texts and literature of standard methods. The quality control & quality assurance studies were conducted in accordance to the WHO., AOAC., IPC. and UPC., approved guidelines. The physicochemical data showed that the drug samples contain, Average values as Foreign Matter, w/w,(%)-Nil, LOD/ moisture (3.85%), Total Ash, w/w,(%)- (6.21%), Acid In-soluble, w/w,(%)- (0.32%), Alcohol and Water Soluble Extractive Matter, w/v, (%)- (2.50%) & (5.56%), pH(1% solution) (5.92), pH(10% solution) (5.35), and various bioactive phytochemical screening examined were assessed in BH., HPTLC. studies of chloroform and alcohol extracts of different samples P1.P2 and P3 of Petha BH. Classical formulation showed various spots at examine under 254nm and under 366nm (UV. region), found no major spots is observed, under exposed to Vinyl - Sulfuric acid reagent. derivatized obtained with equate, best separation using selected suitable solvent system of mobile phase. The quality control studies results revealed the absence of hazardous and toxic contamination from the drug samples of BH. Moreover the obtained research studies data and comparative screening will provide the referential supportive information in the development of Pharmacopeial standard monographs, identification of classical formulation, reinvestigation, quality assurance and pharmaco- vigilance and validation of the Quality standard of BH. classical sweet food providing the quality food used as a medicine to Health & Wellness of needful public mass of the world.