Sugarcane is a wonderful industrial cash crop which is most efficient solar energy harvester and highly stress tolerant. It is planted by stem cuttings (setts) ratooning is the main practice for sugarcane cultivation throughout the world. The productivity of sugarcane under multi-ratooning is declining by 30-50 % every year, due to reduction in cane population per hectare. To find out concrete reasons for this decline an attempt was made to analyze the alterations in physiological, biochemical and enzymological parameters in ratoon cane plants, under monoculturing. To accomplish this objective sugarcane cultivar - Co 86032 from two different sites under ratooning was used.For all analyses standard physiological procedures were used. The results revealed that plant cane had higher contents of proteins and starch than successively increasing number of ratoons while total sugars increased in amount. There was also observed gradually increasing rate of accumulation of different antioxidants like proline and glycine betaine along with ratooning. It had stimulated the activities of antioxidant enzymes like SOD, POD and PPO in cultivar of both fields under monoculturing. The significant changes in physiological, biochemical and enzymological parameters in ratoon cane plants could be assigned to allelobiogenesis stress caused due to synergistic effects of allelochemicals produced during ratooning.
