The aim of this work is to investigate the phytochemical analysis and antimicrobial activities of different crude extracts from rhizome of Curcuma pseudomontana J. Graham. Different organic solvents including hexane, chloroform, ethyl acetate and methanol were used to prepare the crude extracts from rhizome. The extracts of rhizome were tested for their antimicrobial activity by agar well diffusion method, minimum inhibitory concentration and minimum microbial concentration. The phytochemical analysis shows the presence of alkaloid, flavonoid, tannin, steroids, saponins, carbohydrate, proteins, and amino acids in different rhizome extracts. However, not all extracts show presence of these compounds except ethyl acetate. In gram-positive bacteria the B. subtilis was found to be the more sensitive than S. aureus and S. scuiri and in gram-negative, P. aeruginosa most sensitive, followed by the E. coli and K. pneumonia. Among the fungi used, the C.albicans was found to be the most sensitive followed by the A.fumigatus, A.niger and A.flavas. Therefore, in view of these results, the ability of the extracts to inhibit the growth of several bacteria and fungi is an indication of the broad-spectrum antimicrobial potential of Curcuma pseudomontana J. Graham. In conclusion, all organic crude extracts from rhizome can be used as sources of new antimicrobial agents but ethyl acetate extract showed most potential and higher antimicrobial property.
