The search for new antimicrobial agents has accelerated due to the rise in antibiotic-resistant bacterial infections, and green-synthesized silver nanoparticles (AgNPs) have emerged as a viable substitute. The stem extract of Rotheca serrata (L.) Steane & Mabb., a medicinal plant recognized for its bioactive phytoconstituents, was used in this study to create silver nanoparticles. AgNP production was verified by UV-Vis spectroscopy, which revealed a distinctive surface plasmon resonance peak at about 400 nm. The presence of alkaloids, tannins, saponins, and glycosides was verified by phytochemical screening of the stem extract; these compounds most likely helped to lower the silver ions and stabilize the nanoparticles. Using the agar well diffusion method, the antibacterial activity of the biosynthesized AgNPs was assessed against both Gram-positive (Staphylococcus aureus, Bacillus subtilis) and Gram-negative (Escherichia coli, Pseudomonas aeruginosa) microorganisms. Significant antibacterial activities were demonstrated by the AgNPs; at different concentrations.
