Phytochemical Screening And The Antifeedant Effect Of Azadirachta Indica-Leaves, Allium Sativum-Bulbs And Oscimum Sanctum-Leaves On The Larvae Of Chilo Partellus

Research Article
Gaherwal S., Aziz R.U., Kori P., Sahu P and Kavya Manvani
DOI: 
xxx-xxxxx-xxxx
Subject: 
science
KeyWords: 
Phytochemicals screening, antifeedant, Chilo partellus, Azadirachta indica, Allium sativum, Oscimum sanctum
Abstract: 

The present study deals with the antifeedent effect of different plant extracts on Maize stem borer, Chilo partellus. Three medicinal plant material like Azadirachta indica-Leaves, Allium sativum- Bulbs and Oscimum sanctum- Leaves extract were tested against Maize stem borer, Chilo partellus (Swinhoe). The plant extract was prepared by soxhlet extraction method using two solvents like distilled water and ethanol at different percent concentrations (2.0 %-10 %) were used. Phytochemical screening were conducted using standard methods of analysis and the antifeedant effect of plant extract were carried out by using leaf disk choice bioassays. The result of the phytochemical screening was done to check the presence of flavonoids, alkaloids, saponins, tannins and cardiac glycosides. The antifeedant effect of plant extracts were recorded at 12, 24 and 48 hours. All three plant extract in both solvents showed the antifeedant activity. The ethanolic extract of Azadirachta indica (leaves) followed by Allium sativum (bulbs) were found effective and showed the highest antifeedant effect as compared to Oscimum sanctum.10.0 % concentration of Ethanolic extract of Azadirachta indica showed the maximum antifeedant effect (80.33 %) followed by Allium sativum (74.37 %) at 48 hours and the minimum antifeedant (2.09 %) was observed in 2 % aqueous extract of Oscimum sanctum at 12 hours. The concentration of plant extracts increases, the antifeedant effect also increased. Therefore, in general the antifeedant effect of different concentrations, irrespective of the extracts decreased with decrease in concentration from 10 % to 2 %.