Plants are of the best reservoirs of medicinal wealth among the natural medicines. The main aim of this study was to screen the phytochemicals and minerals present in the whole plant of Mollugo cerviana (L.) Ser. Preliminarily all the phytochemicals were extracted using different solvents (Aqueous, ethanol, methanol, acetone, chloroform, and petroleum ether) and screened qualitatively, which showed the presence of carbohydrates, proteins and amino acids, alkaloids, phenols, flavonoids, tannins, terpenoids, steroids, saponins, cardiac glycosides, gums and mucilages and thiols at higher concentration in the ethanolic extract than the other solvents tested. Depending on this, HPTLC, GC-MS, quantitative and mineral analysis were carried out in tha ethanolic extract of Mollugo cerviana. HPTLC analysis confirmed the presence of alkaloids, flavonoids and terpenoids, whereas GC-MS analysis revealed the presence of five bioactive compounds in the ethanolic extract. In addition to this quantitative estimation of this extract showed to contain alkaloids, total phenols, flavonoids, tannins and steroids and among this, total phenols were found to be present at higher quantity. Ethanolic extract of Mollugo cerviana was also found to possess minerals such as copper, zinc and magnesium at considerable amounts. Thus, ethanolic extract of Mollugo cerviana may be helpful for rationale use of this plant in the modern system of health care.