Introduction: Diabetic foot infection is one of the most common complication of diabetes mellitus. It leads to morbidity & mortality in diabetic patients. The Primary requirement for proper management of diabetic foot ulcers is the isolation of organisms & accurate selection of antibiotics. Aim and Objective: (1) To isolate and identify of gram-negative bacteria associated with diabetic foot ulcer patients. (2) To study antibiotic susceptibility patterns for the same. Materials & Methods: Total of 408 samples from wound discharge of diabetic foot ulcer patients were collected with all aseptic precautions & processed according to standard guidelines. Result: Out of 408 proceeded samples, 182 (44.61%) were culture positive, among which 165 (90.66%) were Gram Negative organisms & 17(9.34%) were Gram Positive. Among Gram- Negative Bacteria, Pseudomonas aeruginosa 48(29.09%) was the most predominant organism followed by Escherichia coli 46(27.87%), Klebsiella pneumoniae 33(20%), etc. Among all Gram Negative organism, most of them were sensitive to Imipenem 97.58%, followed by Piperacillin – tazobactam 93.94% and Gentamicin 84.24%. Conclusion: From this study, we can conclude that Gram-negative organisms are the most prevalent organisms which are isolated from diabetic foot infections. These organisms show a variety of sensitivity pattern to the commonly used antimicrobials, which requires prompt antibiotic sensitivity test according to which, the treatment can be streamlined to minimize morbidity & mortality among the patients.
