Increased exposure to Ultraviolet-B (UV-B) radiation is the major risk for various skin injuries. Numerous studies have shown that bioproducts could demonstrate photopreventive efficacy against UV-B damage. Herein, we aimed to investigate the photoprotective effects of cynin (cynoglossus semifasciatus) on preservation of human keratinocytes,and antioxidants, reactive oxygen species (ROS) against UV-B. This study demonstrated feasibility of cynin on cosmeceutical application via ultraviolet B induced damage protective effects such as enzymatic (SOD,CAT,GPX),nonenzymatic (GSH) antioxidants, lipid peroxidation assays and decrease of apoptotic cell death. To investigate protein secondary structural characterization used Fourier transform infrared spectroscop. Secondary structure of skin epidermal mucoprotein (SEM) of C. semifasciatus was alpha-helical, henceforth so named the resultant product as cynin (cynoglossus semifasciatus). cynin pre-trated enzymatic, nonenzymatic antioxidants and lipid peroxidation significantly decreased the effects of UV-B irradiate at 15mJ/cm2 in HaCaT cells in dose dependent manner. The florescent microscopical observation revealed that the cell death was significantly reduced by pre-treated cynin in a dose dependent manner in HaCaT cells when exposed to UV-B irradiated at 15mJ/cm2. Through these results we verified that the cynin has effects on scavenging enzymatic nonenzymatic antioxidants,lipid perooxidation and protection of human keratinocytes against UV-B
