The proteomic analysis for identification of calcium binding proteins (CBPs) could be useful for development of nutraceuticals and food to food fortification. The present investigation was aimed to explore the proteomic study to identify CBPs in major pulses available in Nainital District. Pulses were collected from local market and farmers of Nainital region of Kumaun. All physiological parameters were examined as per standard protocols. Total buffer soluble proteins (TBSPs) were quantified with the help of Bradford assay. TBSPs were checked their quality in discontinuous system of sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) gel with staining Coomassie Brilliant Blue dye. CBPs were examined by the staining with the stains-all staining. In this study the major finding is that the pulses are the rich source of soluble protein (6.57 to 11.18mg/g) and CBPs. One to three distinct bands were observed after stains-all staining of SDSPAGE gel. This is the first hand report on CBPs report of major pulses collected from Nainital of Kumaun region. The study would be useful to the researchers of this field to generate information on nutraceuticals for curing malnutrition including farmers. Present study generated significant information on CBPs including the hope to convert the staple crop in to cash crop to marginal farmers.
