Purification And Characterization Of Invertase From The Midgut Of Fifth Instar Larvae Of Anthereae Mylitta Drury (Daba Tv)

Invertase obtained from fifth instar larvae of Anthereaemylittadrurywas purified by 45-85% (NH₄)2SO₄ fractionation followed by dialysis and DEAE column. The enzyme was purified to 15.32 folds with recovery of 20.1% by DEAE-column chromatography and the molecularweight estimated was 66kDa by SDS-PAGE. Fifth instar larvae of Anthereaemylittadrury (Daba TV) contains high level of invertase at pH 10, temperature 40°C, using sucrose as substrate. Invertase has a Vmax value of 0.938 µmol/min/mg and Km value of 7.68 µmol/min/mg at temperature 40°C and pH 10.The increase in chemical concentration of CaCl₂ and EDTA stimulated theinvertase activity whereas NaCl, Urea, MgCl₂ and ZnCl₂ inhibited the activity.