There is an increasing concern about the exposure to fungal aerosols in occupational environments and associated respiratory allergic diseases and asthma. A large number of people work in cattle sheds around the world, pulmonary function impairments and higher frequency of respiratory symptoms have been reported in dairy farmers; however, it appears that adequate information on the fungal aerosols from the cattle sheds are largely lacking. The study of airborne cultivable fungal spores was performed in 5 different rural indoor cattle shed in Thane district of Maharashtra, India. The aeromycoflora of the cattle shed was investigated by gravity sampling method using selective medium for fungi. A total of morphologically different 15 fungal isolates were obtained. The study revealed the prevalence of higher frequency of Aspergilli, Ustilago spp., Penicilli, Alternaria, and Cladosporium. The result indicates the poor hygienic condition of indoor environment of selected dairy cattle sheds.