Tephrosia purpurea (Linn.) Pers. (Leguminasae), commonly known in Sanskrit as Sharapunkha is a highly branched, suberect, herbaceous perennial herb. The present study was designed to study the effect of various auxins and cytokinins concentrations on in vitro morphogenesis of Tephrosia purpurea explants. Initially, the seeds of Tephrosia purpurea were collected from local areas of Warangal District. The sterilized seeds are inoculated on the MS medium supplemented with various concentrations of GA3. The first and second leaflets are excised and used as effective source of explants and inoculated separately on MS basal medium supplemented, 3-indole acetic acid, NAA (0.1-2.0+1.0-2.0 mg/L) in combination with 6-Benzyladenine (2.5-4.0 mg/L) and 3-indole acetic acid, NAA (0.1-2.0+1.0-2.0 mg/L) in combination Kinetin (2.5-4.0 mg/L) for callus induction and multiple shoot production. Shoots are transferred to MS medium supplemented with IAA in combination with NAA ranging from 1.5-3.5+0.1-2.0 and single IAA 2.5-4.0 mg/L. The number of shoots 34, 30, 28 and shoot length 7.12±0.9, 6.10±0.1, 6.63±1.0 was found high at 3.5+2.0+1.1, 3.4+1.9+1.0, 3.3+1.8+0.9 mg/L-1 of Kinetin+IAA+NAA respectively. The highest root number 52 and its length 7.21±0.3 was recorded at 3.0+1.5 mg/L-1 of IAA and NAA respectively.
