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Background: Terminalia arjuna (TA) is a native plant of India and South East Asia and has been traditionally used as a cardio protective agent included in Charka Samhita and Astang Hridayam. The bark of this plant had used in many ancient Indian medicinal literature including claiming treating diabetes, obesity, potent lipid lowering and antioxidant activity.Aims of the study: The present study was evaluated the protective effect of bark of TA on dehydration induced uremia and oxidative stress in male Wister strain albino rats. The study has been designed mainly to search out which was the most effective dose of methanol fraction from bark of TA could reduce oxidative stress and uremia of said experimental rats.Materials and Methods: Thirty six male Wister strain albino rats (n=6 rats/group) were administered 4 ml water / 24 hrs interval for 15 days experimental period. Out of 6 groups, 1 control |
and 5 dehydration group (1 group without treatment and another 4 group’s treatment with 10, 20, 40 and 80 mg / kg / day of methanol fraction bark of said plant part). Thin Layer Chromatography (TLC) and High Performances Liquid Chromatography (HPLC) were performed to identify the methanol fraction of bark of TA.
Results: Water deprivation for 15 days showed a significant elevation in the level of blood urinary nitrogen (BUN-urea and creatinine), increased the levels of free radicals like malondialdehyde (MDA) and conjugated dienes (CD) along with significant diminution in the activities of superoxide dismutase (SOD) catalase (CAT) and peroxidase (Px) in kidney tissues which were protected significantly after coadministration daily orally 10-80 mg / kg / day of methanol fraction bark of said plant and were also corrected the level of blood nitrogenous end products to normal level. Beside this methanolic extract was played the most crucial role to correct the uremia and oxidative stress. By the phytocompounds analysis using TLC and HPLC, identified the purified fractions of bark of Terminalia arjuna contains three major phytocompounds, which have capability to control uremia and oxidative stress.
Conclusion: The results suggested that dehydration induced oxidative stress and uremia on male rats might be protected by using above mentioned medicinal plant methanol fraction and also had no toxic effect on blood and kidney by the measurement of glutamic oxaloacetic transaminase and glutamic pyruvic transaminase activities.
