Soil enzymes play a vital role in catalyzing several important reactions necessary for life processes of microorganisms in soil. Soil enzymes play an important role in catalyzing reactions for the decomposition of organic matter and nutrient recycling in ecosystems. Microorganisms are the producers of enzyme activities in soil. Pesticides and Biopesticides, was assessed for its effect on different enzymatic activities (Protease, Cellulase, Amylase, Alkaline phosphatase, Glucosidase and Invertase) in soil for definite period of incubation. Variations in activity were independent upon the period of incubation. An increasing trend in the activity of microbial population and soil enzyme activity were observed during 14th - 28th day of incubation in both control and treated soil. These fluctuations in activities were in accordance with soil pH and organic matter.