In vitro pollen germination of Scoparia dulcis L. belonging to Scrophulariaceae has been carried out to study the role of different nutrients like sucrose, boric acid, different salts like calcium nitrate, potassium nitrate and magnesium sulphate on pollen germination and tube growth. Flowers open in the morning (06:30-07:30 hrs.) after which anther dehiscence takes place. Maximum 97 % pollen germination along with 1183 µm pollen tube development was observed in 5 % sucrose solution supplemented with 100 ppm boric acid. Among the salts, maximum 52 % pollen germination along with 325 µm pollen tube development was observed in 300 ppm calcium nitrate solution. Pollen grains which were collected during anthesis (08:00-09:00 hrs.) showed the best results.
