Alkannin, a red-purple dye and bioactive compound found in the roots of Arnebia hispidissima. It has antibiotic and anti-inflammatory properties and also used in cosmetic and textile industries. The present communication demonstrates the establishment of root cultures of A. hispidissima with the aim of optimizing the production of alkannin. The maximum number of adventitious roots (4.2 per culture bottle) could be initiated from the in vitro regenerated shoots on half strength MS medium containing 1.0 mg L-1 Indole-3 butyric acid (IBA). Highest alkannin content and profuse rooting was also recorded on this media combination. Production of alkannin was influenced by the different culture media. The present investigation may be applicable in designing systems for the large-scale cultivation of A. hispidissima roots for the production of alkannin
