Augmenting bacterial resistance to chemical antibiotics and their probabilistic side effects cause popularity of medicinal plants, so there is an instantaneous and steady need for novel antibacterial compounds from plants. As we know, there is no documented proof on antibacterial effects of Allium sativum (A. sativum) in west of Iran. The aim of the current study was evaluation antibacterial activities of essential oil of A. sativum against Bacillus subtilis(B.subtilis) in west of Iran (in Kermanshah). The antibacterial properties of A. sativum essential oil was evaluated by macro-dilution method in Mueller-Hinton broth medium, agar disk and well diffusion methods. The results illustrated that the essential oil of A. sativum have inhibited the growth of B. subtilis and destroyed it. Also, by increasing the concentration of the A. sativum essential oil, the inhibition zones increased. We believe that the article provide support to the antibacterial effects of the essential oil. In fact, the results indicate that the essential oil of A. sativum can be useful as medicinal or preservative composition. Fractionation and characterization of active molecules will be the future work to investigate.
