Objective: To estimate and correlate serum iron, salivary iron and blood glucose levels in patients with diabetes mellitus. Methods: Study group consisted of 15 diabetics and control group consisted of 15 non-diabetics. Blood sample of 2ml and salivary sample were collected from both the groups. Serum and salivary iron levels and serum glucose levels were estimated using colorimetric method with the help of reagents and semi auto analyser. Pearson’s correlation coefficient test was applied to assess the correlation between salivary and serum iron levels. Results: Results showed significant (P < 0.001) positive correlation between serum and salivary iron levels in diabetics where as in control group, though results showed positive correlation it was statistically non- significant. Conclusion: Salivary and Serum iron levels showed positive correlation with significantly high levels in case of Diabetes Mellitus. Hence saliva can be used as a diagnostic tool to demonstrate the iron overload.