A total of 280 unsexed broiler chicks were distributed randomly in seven dietary treatments (D). The diets were D1: basal diet + mineral mixture without Zn; D2: D1 + 15ppm inorganic Zn; D3: D1 + 15ppm organic Zn; D4: D1 + 7.5ppm organic Zn; D5: D1 + 0.3ppm nano Zn; D6: D1 + 0.06ppm nano Zn; D7: D1 + 0.03ppm nano Zn. Blood samples were collected from four birds of each group on 21st and 42nd day of the study. The different serum biochemical indices were spectrophotometrically measured using the chemical kits prepared by Crest Biosystems. The lowest serum cholesterol was found in group D6 which differed significantly (P<0.05) from D1 and D7. The highest level of SGPT was found in D7 which varied significantly (P<0.05) from D1, D2 and D4 and the lowest level of SGPT was observed in group D1 which varied significantly (P<0.05) with all the other treatments except D2. The highest average level of ALP was observed in treatment D5 which varied significantly (P<0.05) with all the other treatments except D6 whereas its lowest level was observed to be present in D1 which was significantly (P<0.05) different from all the remaining dietary groups. Organic and nano zinc supplemented @ 15 ppm and 0.06 ppm, respectively to the basal diet altered the serum cholesterol, SGPT and ALP levels significantly (P<0.05).