Acetylcholinestrase is one of the most important enzymes in nervous system, and plays a role in the signal transduction in the somatic nervous system by termination of signal transduction in the synapse. It has been reported that the function of this enzyme plays a role in Alzimer's disease. Selenium is one of the most important micronutrient. Many investigations have been performed about the physiological, biochemical and behavioral effects of this element, such as postponing the Alzheimer's symptoms in the elderly and delaying the initiation signs of skin aging. Recent studies have shown that this element protects various enzymes against the toxicity caused by heavy metals such as; Pb, Al, Cu and Cd. Previous study demonstrated that Acetylcholinestrase activity was decreased under the influence of extremely low frequency electromagnetic field. In this study the effects of different concentrations of Sodium selenate (0, 390, 870, 1300 µM) on Acetylcholinestrase activity under 217 Hz, 300 µT investigated using UV-visible spectroscopy, fluorescence spectroscopy and circular dichroism spectroscopy. The main inactivation take place at a concentration of 1300 µM of Sodium selenate (p<0.05). The results have shown that under 217 Hz, 300 µT by increasing the cocentrations of sodium selenate the enzyme activity declined. Also, significant structural changes occurred in the secondary and tertiary structures of Acetylcholinestrase. In conclusion, according to changes observed in the secondary and tertiary structure of enzyme it is proposed that under 217 Hz, 300 µT, Sodium selenate are able to affect the activity of the Acetylcholinestrase Therefore, we suggest that the Sodium selenate could be useful in treatment of Alzimer's disease.
