The present study was undertaken to standardize the methods of propagation for some threatened medicinal plants of North East India. Three endangered species of medicinal plants of North East India viz. Smilax china, Plumbago indica and Rhynchostylis retusa were selected for the study. The results indicated that Stump cutting of Smilax china and Shoot Tip cutting of Plumbago indica recorded the shortest days to root initiation in 19.67 and 12.33 days, shoot emergence in 18.33 and 7.38 days and to attain 30cm height in 54.50 and 129.00 days, respectively. The study also revealed that the highest number of roots per cutting after 6 months both in Smilax china and Plumbago indica were 27.17 and 13.67 numbers respectively. In Rhynchostylis retusa, Cocohusk block + Charcoal bed (under shade net) gave good results in producing number of leaves per plant and roots per plant after 8 month were 7.33 and 7 numbers respectively.
