Catfish of the family plotosidae was extensively rampant all the way through the watercourses of India that let liquid flow away into the Indian Ocean. Catfish species are endemic to the most extreme freshwater environment of the Parangipettai and possess a toxic mucous-covered sting, responsible for most of the injuries, as well as a protective epidermal secretion. The envenomation causes immediate, local and intense pain, soft tissue edema, and a variable extent of bleeding.The present study was carried out on studies on Bioactive properties of mucus and spine extract from catfish Plotosus canius. The crude was extracted with three different solvents aqueous, acetone, chloroform and it is screened for antimicrobial properties and were tested against 6 pathogenic bacteria and 5 pathogenic fungi. The result of present investigation reported that spine and mucus extract of catfish having significant antimicrobial activity. In particularly mucus extracts showed much activity than spine extracts. The crude extract was partially purified by using DEAE cellulose. The antioxidant property of the extract was assessed by 2, 2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method .The toxic activity of crude extracts were determined by illeal loop in chick respectively. The haemolytic activities in chick, goat, and human blood erythrocytes were recorded. The result reported that mucus extracts showed a very strong haemolytic activity than spine extracts. The estimation of protein gist were intervened by SDS –PAGE and the results showed that all the extracts showed significant number of protein bands in range from 7 KDa to 21 KDa respectively. It could be surmised from the contemporary study that Plotosus canius venom boasts a assorted mixture of peptides, protein and pharmacologically active components.
