Eclipta prostrate was sequentially extracted with hexane, chloroform and ethyl acetate three solvents separate the compounds on the basis of their polarity. The extracts were analyzed to delineate their chemical structure and properties using High performance Thin Layer Chromotography (HPTLC), Gas chromatography-Mass spectrometry (GC-MS) and Liquid Chromotography Mass Spectrometry (LC-MS) techniques. The separation of the various compounds in the crude extracts and phytochemical studies by elucidating their structures. HPTLC has revealed the presence of about 16 compounds in the hexane extract. Through GC-MS about 28 compounds have been characterized in the chloroform extract and about 32 compounds have been characterized in the Ethyl Acetate extract. Structural elucidation has been done on 11 compounds present in the Ethyl acetate extract with the help of LC-MS. The identified compounds were validated the anticancer property of the plant was tested with EAC tumor cell line using MTT assay for the inhibition of cell proliferation. Testing with all the three extracts indicate that the anticancer property is present in all the three extracts.
