Two orange pigments have been produced by two different bacterial strains. They have been isolated from soil of West Bengal, India. Upon 16s rRNA analysis, one strain named as PNSMO has been identified as Pseudomonas geniculata deposited at NCBI with GenBank accession number, KU726551. Another one named as PNSLO has been identified as Deinococcus soli with GenBank accession number, KU726552. Optimum temperature and pH have been adjusted for maximum pigment production. Moreover, pigment productions by the two bacteria have also been increased by using Magnesium Chloride (MgCl2) as the catalytic agent. The two orange pigments have been extracted from two different bacteria by methanol extraction procedure. Those pigments have been identified as Carotene, when passed them through HPLC and UV-Vis spectrometer. The Rf values of those pigments have been found to be around 0.88. It is probable that the carotene pigments, produced by those two bacterial strains, have been reported for the first time by us. In the present investigation, an attempt has been made to find the characteristics of the two orange pigments produced by the different bacterial strains isolated from soil. The results have been tabulated, shown graphically, and discussed.
