Cleome rutidosperma is traditionally used in Indian ayurvedic medicine for the treatment of wide number of health disorders. Plant tissue culture techniques are considered as easy and consistent methods for rapid propagation of plants, especially rare and endangered plant species. In vitro propagation of C.rutidosperma was investigated to develop reliable protocols for direct shoot regeneration induced by culturing nodal explants and comparison the components between wild and micropropagated. The nodal explants were cultured on MS medium supplemented with different concentration of plant growth regulators like 6-Benzylaminopurine and NAA. The bioactive compound of chloroform extracts was identified by Gas chromatography–mass spectrometry method. Multiple shoot generation was achieved after 28 days of incubation with average five shoots per explants. The wild and micropropagated plant showed the presence of eleven and nine compounds respectively. The study concluded that callus formation yield mass multiplication and chloroform extracts showed more compounds than that of micropropagated plant.
