DNA hairpins are sensitive probes for oligonucleotides identification that discriminate between two sequences over single base pair change. When these hairpins are immobilized onto surface this not only increases their sensitivity but also provides new path in development of microarray based biosensor. Current work includes molecular beacon based biosensor development for hypertensive SNP rs699 recognition. Hybridization efficiency was found to be higher in rs699 mutation complement probe than in non-compliment one. The developed biosensor has response time of 5 min and optimum working temperature of 35ºC. Immobilization of oligonucleotide was cross confirmed by electrochemical analysis of surface via electrochemical Impedance, Cyclic Voltammetry and Differential Pulse Voltammetry. Further biosensor could be applied to clinical hypertensive and normotensive patients, to ensure maximum efficacy of drug and minimal adverse effect.
