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Environmental, economic and political pressures have driven the interest towards the search of sustainable feedstock for bio fuel production. At present, macroalgae (green, brown and red macro algae) is getting growing consideration as an alternative resource for sustainable biomass to produce bio fuels, biochemical and food. The unique chemical composition and wide variation in the availability create various opportunities and also challenges for bio-based energy production. Recently, numerous studies have taken place in the exploitation of seaweed as carbon sources for the bio ethanol production. Thus, this paper attempts to highlight the characteristics, processing techniques and potential applications of the macroalgae. The Current study fresh water macroalgae as an alternative raw material for the biodiesel production. The obtained results show that biodiesel production from oil extracted from fresh algae is feasible by transesterification. Oil extraction can be carried out simultaneously with the transesterification. To investigate the optimum reaction conditions, the reaction was carried out at various methanol to oil molar ratios, catalyst concentrations and reaction temperatures. Moreover, the properties of macro algae transesterification residue after transesterification were analyzed, concluding that it is a suitable material for fuel manufacturing.