This study was conducted to explore the possibility of biosynthesis of gold nanoparticles by using the fungus Fusarium oxysporum. The cell filtrate of Fusarium oxysporum reacted with HAuCl4 ions, resulting formation of gold nanoparticles within 3 hours. The gold nanoparticles were characterized by Visual analysis, UV-Vis absorption spectroscopy and Transmission electron microscopy (TEM). The gold nanoparticles exhibited maximum absorbance at 550 nm in UV-Vis spectroscopy. UV-spectral reading clearly showed that stability of gold nanoparticles also constant after 3 month. TEM showed polydisperse spherical and ellipsoid nanoparticles in the size range from 18-24 nm and average size 21.82 nm.T he AuNPs were evaluated for their impact on viability of seed. F. oxysporum synthesized gold nanoparticles observed optimistic effect on seed germination. Therefore biosynthesized gold nanoparticles have biological assay used in agricultural purposes to increases the viability of seeds.
