tinospora cordifolia (willd.) miers ex hook. f & thoms. (menispermaceae): rapid in vitro propagation through shoot tip explants

Shoot tip explants were employed to develop a method of rapid in vitro multiplication of Tinospora cordifolia, an important plant for Ayurveda and medicine. Multiple shoot were induced on MS medium and woody plant medium supplemented with different concentrations of cytokinins (BAP and KIN). Maximum number of shoots was induced in woody plant medium supplemented with 2 mg/l BAP. In vitro rooting was achieved on half strength MS medium supplemented with IBA and IAA, with maximum rooting with 1.0 mg/l IBA. The shoots with well-developed roots were successfully transferred to field with 70 % survival.