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Background: CHLORHEXIDINE is one of the widely used gold standard antiplaque and antigingivitis agent. Lack of knowledge on the mechanism of action of chlorhexidine and its adverse effects has led to the injudicious use of chlorhexidine. The practice of chlorhexidine prescription by dental practioners has to be improved. Aim and objective: The present study was carried out to assess the knowledge, attitude and usage practice of chlorhexidine mouthrinse among the dental practioners in Erode and Namakkal districts Materials and methods: A questionnaire of 15 multiple choice questions were given to general dental practitioners in Erode, Nammakkal and Bhavani districts. A total of 120 dentists participated in the study whom were divided into 4 groups based on the years of experience. Group A included dentist with 1-2 years of clinical experience, while group B,C and D included 2-5 years, 5- 10 years and more than 10 years respectively. The questions were about the prescription of chlorhexidine mouthrinse in the regular dental practice. Statastical analysis of 15 questions were made. Results: A overall analysis suggest that all dentists who participated in the study have good knowledge about prescription of chlorhexidine in regular dental practice. But the knowledge was good with increasing years of experience. Among all the groups in general a lack of awareness was observed with regard to brushing and chlorhexidine use. Conclusion: The present study shows adequate knowledge and awareness on chlorhexidine prescription and use among dental practioners.