The Use of Natural Administration Technique As An Alternative to Orogastric Gavage Prevents Undesirable Changes In The Results of Acth Plasma And Antibodies Due To Stress In Rats

Orogastric gavage is a common laboratory method in toxicology and pharmacokinetic studies, where it is commonly used for daily dosing of rats, mice, rabbits, and monkeys. This work performed on Wistar rats showed that the repeated use of this technique, unlike the natural administration directly from the syringe to be treated with a dose of 1 ml/kg of the placebo (NaCl 0 9%), triggers in rats undesirable stress for the success of the experiment expressed by a significant increase in ACTH levels that cause immuno suppression of detected antibodies.