In the present work is to established simple, accurate, rapid and validated (as per ICH) RP-HPLC method developed for the quantification of Triamcinolone acetonide tablet and injection. In RP-HPLC method the column used was Phenomenex Luna C18, Column (250 mm x 4.6 mm id; 5 µm particle size) and the mobile phase was composed of Acetonitrile: 0.05M Phosphate buffer pH adjusted to 6.8 using NaOH with 0.1% of Triethylamine (55:45 v/v) with flow rate 1ml/min. Eluents were monitored by UV detector at 238 nm. Calibration curve was linear in the concentration ranges 10-50 µg/ml (r2=0.9998) for TCA. The percentage purity of the TCA tablet and injection was found to be 99.48 ± 0.0203 and 99.48 ± 0.0076 respectively. The precision of the method was confirmed by the marketed formulation for six times. The % RSD was found to be 0.0204 and 0.0077 for TCA. The % RSD for tablet and injection accuracy was found to be 0.0127 and 0.0034 for TCA.
