The enzyme secretory phospholipase A2 is responsible for the hydrolysis of membrane phospholipids that release arachidonic acid, which serves as a substrate for pro-inflammatory mediators, such as prostaglandins and leucotriens. The plant bio active compounds inhibiting PLA2 possibly implicate as potential therapeutic agents in the treatment of inflammation related diseases. In the present study Gymnospora montana subjected to inhibition of sPLA2 along with in vitro anti- oxidant (DPPH scavenging), Anti-lipid peroxidation activity & lipoxygenase inhibition. Among different solvent extracts G. montana methanol extract shows grater phytochemical contents along with anti- oxidant activity. The G. montana methanol extract exhibit Greater DPPH free radical scavenging activity of 82.03%. The reducing power and anti-lipid peroxidation activity was found to be 80.34% and 78.44% respectively. Further methanol extract of G.montana shows 63.24% of HPF-PLA2 inhibition at 75μg concentration and inhibited 15-LOX enzyme in concentration dependent manner. IC50 value of methanol extract of G. montana was found to be 64.86 μg/μL. The present study may lead to the better understanding of PLA2 inhibition by phytochemicals. This may help to develop better anti-inflammatory drugs
