Honey is one of the main products of honeybee species, widely used as food throughout the world. India produces different types of honey which shows specific physico-chemical properties assuring acceptable quality as prescribed by the IHC. However, published reports on the physical properties and chemical constituents of unifloral and multifloral honey are poor and in the present investigation five physical properties and six chemical constituents were analyzed by following standard methods. Most of the physical properties viz., pH, specific gravity, electrical conductivity, absorbance, turbidity, and chemical constituents such as two monosaccharide’s (fructose and glucose), proteins and ten minerals excepting moisture content and three vitamins revealed a significant difference between unifloral and multifloral honey in southern Karnataka. Results from the present investigation clearly indicated that forage collected by honeybees from flora available at diversified agro-climatic locations exhibit specific physical properties and chemical constituents in honey and which is unique to unifloral and multifloral honeys.